Journal: Frontiers in Microbiology

Article Title: Evaluation of the Immunochromatographic NG-Test Carba 5, RESIST-5 O.O.K.N.V., and IMP K -SeT for Rapid Detection of KPC-, NDM-, IMP-, VIM-type, and OXA-48-like Carbapenemase Among Enterobacterales

doi: 10.3389/fmicb.2020.609856

Figure Lengend Snippet: Reference strains used in this study.

Article Snippet: ATCC BAA 2341 , E. cloacae , KPC-2 , KPC , KPC.

Techniques:

Journal: Data in Brief

Article Title: Data on human neutrophil activation induced by pepducins with amino acid sequences derived from β2AR and CXCR4

doi: 10.1016/j.dib.2016.05.065

Figure Lengend Snippet: The CXCR4 pepducin ATI-2341 triggers a direct activation of the neutrophil NADPH-oxidase through FPR2. A) ATI-2341 dose-dependently triggers neutrophil superoxide production, as measured by isoluminol-amplified chemiluminescence. Data are presented as normalized peak response with the fitted curves. The EC 50 value and the 95% confidence interval were calculated from 8 independent experiments. Inset: A representative ATI-2341 (1 µM) induced neutrophil response is shown. Abscissa, Time of study; Ordinate, Superoxide production (arbitrary units). B) Desensitized neutrophils incubated for 5 min at 37 °C without (solid lines) or with 1 µM ATI-2341 (dash lines) were activated with the FPR2 specific agonist F2Pal 10 (1 µM; main figure) or the FPR1 specific agonist fMLF (100 nM, inset). Arrows indicate the time point for agonist addition. C) Neutrophils were pre-incubated for 5 min at 37 °C without any additive, with either of the two FPR2 specific inhibitors PBP10 (1 µM) or HF965A (1 µM), or with the FPR1 specific inhibitor cyclosporin H (CysH, 1 µM). The neutrophils were then activated with ATI-2341 (1 µM) and superoxide production was recorded by isoluminol-amplified chemiluminescence. The results are expressed as percent inhibition compared to the activity induced in cells incubated without any inhibitor (mean±SD, n =7). N.I. equals no inhibition.

Article Snippet: The FPR2 pepducin F2Pal 10 (Pal-KIHKKGMIKS, amino acid sequence from a part of third intracellular loop of FPR2), the β 2 AR pepducin ICL3-8 (Pal-LQKIDKSEGRFHV, amino acid sequence from a part of the third intracellular loop of β 2 AR) , and the CXCR4 pepducin ATI-2341 (Pal-MGYQKKLRSMTDKYRL, amino acid sequence from the first intracellular loop of CXCR4) were from Caslo Laboratory (Lyngby, Denmark).

Techniques: Activation Assay, Amplification, Incubation, Inhibition, Activity Assay